Sporeless Oyster Liquid Culture


Looking for the best Sporeless Oyster Liquid Culture around? Our Liquid Culture is carefully monitored until the solution is 90% colonized, then pulled to keep it fresh in the syringe until it gets to you!

Species Pleurotus ostreatus var. Sporeless
Difficulty ℹ️ ?
Spore Coloration White
Ecology Saprotrophic
Edibility Choice


COMPOSTING. Wheat straw is chopped at 3-5 cm and wetted. Moisture must reach 72-74%, beware not to lose too much structure to keep the stack aerated. Periodically straw is wetted and mixed with other N rich raw materials to balance C/N ratio and conditioners to balance pH. Be sure the stack is mixed homogenous. After wetting the substrate needs a pasteurization at 58-62°C for 10-12 hours or more (max 70°C – 36 hours) and then Conditioned to 48-50°C to reduce the ammonia and increase selectivity. Pasteurized substrate must have 70-71% moisture, pH 7,0-7,6 and Ntot 0,8-1,3% (dry weight). N contents depend on the raw material used: usually N rich substrates are produced in winter while full straw substrate are produced in summer.

INCUBATION. Substrate is spawned at 2,0-2,5% then packaged in 15-20 kg blocks. Keep the temperature at 27-29°C. Mycelium will grow aggressively and after 12-18 days it will be fully grown. Consider Pleurotus mycelium grows best at 28,5°C, but do not exceed the growth temperature!

PINSETTING. As optimum values air T° must fall on range 14-17°C and by humidification R.H. is kept 95% to avoid drying pins. CO2 levels around 1.000 ppm depending on the demand for number of pins per cluster. 100 lux for 12 hours a day is enough. First primordia appear at 15-20 days. More light and cold temperatures will darken the fruit bodies extra.

FRUITING. When primordia reach 1,5-2,0 cm diameter room temperature must be increased to 16-17°C, CO2 under 700-900 ppm and RH% lowered to 85-90% to stimulate evaporation. After the first flush CO2, R.H. and substrate temperature need to go up in order to get a good new pin-setting. Decrease again when the pins are set.

Some photos of this product in its wild-foraged form are sourced from iNaturalist or Wikipedia, by mikebishop21, kastik-talsu, Fluff Berger, Keith Seifert, Jake McCumber, and others. Licensed by CC-BY-SA.

Frequently Asked Questions

Mushroom Liquid Culture is a nutrient solution with live mycelial bodies suspended in it. It comes in a filled 10cc syringe with a needle, and is entirely sterile!


Interested in making your own?! We offer our proprietary Liquid Culture mix right here

It sure is! Mushroom spores are the microscopic “seeds” of mushrooms.

Liquid culture is when those seeds have “taken root”, so to speak! Mushroom spores are very sturdy, and well suited to storage and transport.

Liquid culture offers you a way to add rocket fuel to your gourmet grows, and get to your desired fruiting bodies much faster!

Looking for spore syringes, instead of liquid culture? Head over here!

Keep your liquid culture ideally in the fridge for up to a year. You can also keep it in a cool dark place for 3~6 months.

Get yourself some substrate, an agar plate, or whatever medium you choose, attach the needle provided in your kit, and inject a small amount into your chosen medium!

If you any questions as to the density, sterility or other concerns about your order please reach out as soon as possible through our Help Center.

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Generally speaking, liquid cultures ships on the next shipping day. Check the calendar here!

Each kit comes with 10cc of sterile, live mycelium in nutrient solution and a single 18Ga needle for use.

Some photos on this page have been sourced from iNaturalist or Wikipedia, taken mark-groeneveld, Alan Rockefeller, Matthew Borella, Denis Zabin, kaju, Andrey Loria, Juan Carlos Pérez Magaña or others not yet added to this list. See a photo your recognize that isn’t properly credited? Get a giftcard for letting us know!

Can’t wait to get your hands on your spore syringes or liquid cultures? Head over to our help center to get the most up to date information on your order.

Customer Reviews

Based on 1 review
Clean syringe, grows well in my petri dish

This is a good clean culture, will look forward to buying more jn the future.

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Sporeless Oyster Liquid Culture

Availability: In stock